在共轭亚油酸高效液相色谱法分析中,通过单因素试验和响应面分析法,研究了NaOCH3-CH3OH甲酯化分析奶样中CLA的最佳条件。在单因素实验的基础上,以Box-Behnken响应面分析法(三因素三水平)优化甲酯化条件。实验结果表明:最适衍生化条件为NaOCH3-CH3OH添加量为8.56mL,温度为24.4℃,时间为12.8min。并以该条件对液态奶中的9c,11t-CLA含量进行了测定。

In this paper,the base-catalyzed conjugated linoleic acid methyl ester preparation was evaluated by the response surface methodology (RSM).Based on the single factor experiment,the optimum methylation conditions were obtained with Box-Behnken design (BBD).The results showed that the optimum conditions for the methylation of conjugated linoleic acid were as follows:reaction temperature 24.4℃,reaction time 12.8 min,0.5 M sodium methoxide-methanol 8.56 mL for methylation.