制备海藻酸钠-壳聚糖-海藻酸钠(ACA)液芯微胶囊,考察了小分子物质从溶液向ACA液芯微胶囊内的传质性能并计算扩散系数;确定ACA液芯微胶囊膜的截留分子量。比较游离培养、固芯微囊化和液芯微囊化培养对酿酒酵母细胞生长的影响,并考察细胞在液芯微胶囊内的代谢情况。结果表明,小分子物质进入ACA液芯微胶囊的扩散系数(mm/min)为谷氨酸(0.0126)、L-苯丙氨酸(0.0110)、葡萄糖(0.0071)和乳酸(0.0049),膜的截留分子量为2000。液芯微胶囊中细胞的生长速度和密度均优于固芯微胶囊,液芯微囊化酵母细胞能够进行多批次连续培养,产量稳定。

ACA microcapsule with liquid core (LCM) was prepared for studying the diffusion coefficient of low molecular weight substances and determining molecular mass cut-off (MMCO) of the membrane.The growth of the Saccharomyces cerevisiae was studied,which were encapsulated into LCM and solid core ACA(SCM).Free cell culture was used as control.The behavior of cell glucose consumption and ethanol production were determined.The result shows that the diffusion coefficients (mm/min) for low molecular weight substances to ingress though the LCM were 0.0126 for glutamic acid,0.0110 for L-phenylalanine,0.0071 for glucose and 0.0049 for lactose.The MMCO is 2000.Microencapsulated cells with LCM has higher growth rate than that with SCM.Ethanol production in batch culture of Saccharomyces cerevisiae encapsulated into LCM maintain high rate.