采用蒸汽爆破、超微粉碎、纤维素酶水解预处理技术对啤酒糟进行了预处理,分析了预处理前后啤酒糟纤维形态结构、纤维组分和还原糖的变化,结果表明,蒸汽爆破预处理技术能有效的破坏啤酒糟中的纤维形态结构,降低中性洗涤纤维(NDF)含量,并提高啤酒糟中还原糖的含量;研究并优化了纤维素酶水解啤酒糟的最佳条件是:酶浓度180U/g,底物浓度为10%,温度为50℃,作用时间为6h;蒸汽爆破后的啤酒糟经纤维素酶水解后,还原糖净增量达12.77mg/mL,NDF基本没有变化。

In this paper,steam-explosion,ultra-fine pulverization,cellulase hydrolysis pre-treatment methods for the brewer's spent grains(BSG)were investigated,and a series of parameters,such as fiber structure,fiber complex,and reducing sugar change,were analyzed.The comparison of fiber structure with pretreatment and without led to the conclusion that the steam-explosion technique can destroy fiber structure,decrease neutral detergent fiber (NDF) content,and increase reducing sugar content.The optimized procedures of cellulase hydrolysis of BSG are as follows:enzyme concentration 180U /g,substrate concentration 10%,temperature 50 ℃,and cellulase hydrolysis time 6h.The increase in content of reducing sugar was amounted to 12.77mg/mL by way of steam-explosion BSG technique and cellulase hydrolysis.However,there was little change in the content of NDF.