以MRS为选择培养基,从腌渍食品中分离得到了15株乳酸菌,并进行了初步的筛选和鉴定。采用纸片扩散法(K-B)研究了乳酸菌对常见5种抗生素的耐药性。发现分离的乳酸菌对氨苄青霉素的耐药率较高,为40%(6/15),对四环素、庆大霉素和链霉素的耐药率较低,各发现1株耐药菌株,所有菌株对头孢噻肟均是敏感的。8株耐药菌的滤膜接合转移试验结果表明,3株乳酸菌可以实现耐药性质粒对大肠杆菌受体菌株的转移。

15 lactic acid bacteria strains were isolated from pickled food on the selective culture medium MRS,and preliminarily identified and screened in this paper.The susceptibility tests of lactic acid bacteria to five kinds of drugs were conducted by K-B method.The results showed that percentage of ampicillin resistance strains was 40% (6/15),one isolate was resistant to tetracin,one isolate was resistant to getamicin,one isolate was resistant to steptomycin and no isolate was resistant to cefotaxime.The conjugation tests of 8 resistant strains demonstrated resistant plasmids of 3 lactic acid bacteria strains can be transferred to recipient E.coli RG488.