为提高枯草芽孢杆菌使用效果,以海藻酸钠、明胶为壁材,采用静电喷雾法制备了枯草芽孢杆菌微胶囊。通过正交试验,得到微囊化枯草芽孢杆菌的较优制备工艺为:芯壁材比1:5,明胶浓度0.5%,海藻酸钠浓度2%,微胶囊的包埋率可达90.7%。制得的微胶囊具有一定的耐酸性和较好的肠溶性。经恒温储藏3个月加速实验,结果表明:微囊化枯草芽孢杆菌活菌数约为菌液活菌数的130倍,且存活率远高于菌液的存活率。

In this study,we adopted sodium alginate and gelatin with good biocompatibility as wall material,and prepared Bacillus subtilis microcapsules using electrostatic spray method.Better preparation of microencapsulated experimental conditions was determined by orthogonal test as follows:proportion of Bacillus subtilis and gum was ratio 1:5,gelatin concentration of 0.5% sodium alginate concentration of 2%,and the rate of microcapsules embedded up to 90.7%.The obtained microcapsules have good enteric solubility and acid resistance.The 3-month-accelerated-temperature storage experiment showed the following results:the aerobic bacterial count of microcapsules is about 130 times the number of bacterial suspension;the survival rate of microencapsuled Bacillus subtilis is much higher than those un-microencapsuled Bacillus subtilis.