研究大孔树脂从茄子叶的提取物中分离纯化茄尼醇,并以RP-HPLC法测定茄尼醇的质量分数。以5种树脂对茄尼醇作静态吸附和解吸,吸附量和解吸率为考察指标,选择最佳的树脂;动态解吸过程中,采用正交实验考察解吸剂浓度配比、解吸流速及时间对茄尼醇质量分数和实测收率的影响。结果表明:HPD300对茄尼醇的吸附量为61.23mg/g,解吸率为85.38%,优于其他4种树脂;HPD300做动态解吸时,最佳条件为:先用体积分数50%的乙醇将极性偏大的杂质洗脱完全,然后以体积分数80%的乙醇快速洗脱茄尼醇,流速为3mL/min,解吸时间为100min,茄尼醇质量分数为67.23%,实测收率在91%以上。

Solanesol was isolated and purified from the extracts of eggplant leaves by macroporous resins,and RP-HPLC method was established for determining the content of solanesol.The static adsorption and desorption of solanesol on five kinds of macroporous resins was performed and the static adsorption capacity and desorption ratio were used to evaluate the best resin.The results showed the static adsorption capacity of HPD300 was 61.23 mg/g,and the desorption ratio was 85.38%,better than the other resins;when HPD300 was used as dynamic desorption,the optimum conditions were:50% ethanol completely rinsed strong polar components first and then followed by 80% ethanol quick rinse at 3 mL/min flow rate.The dynamic desorption time was 100 min.The content of solanesol was reached to 67.23%,and the recovery was higher than 91%.