研究了利用高效液相色谱法对水产品中的ATP关联化合物进行检测,确定了缓冲液的最佳pH值、流速和浓度,并对方法的准确度、精确度和检测限进行了测定。实验结果显示:pH值6.5,流速0.70mL/min时色谱峰的分离情况较好。7种ATP关联化合物含量在2～500μg/mL时,浓度与色谱峰面积呈良好的线性关系。本方法的相对标准偏差为0.08%～6.81%,加标平均回收率为98.11%～102.25%。方法检出限为52～53ng/mL。

In this study,the detection method of ATP related compounds of fishery products by using HPLC was researched.The optimal conditions such as pH value,flow rate and concentration of mobile phase were determined.The accuracy,precision and limit of quantitation were surveyed and evaluated.The results showed that when the pH value was at 6.5 and the flow rate 0.70 mL/min the seven chromatographic peaks were well separated.When the concentration of seven ATP related compounds were from 2 μg/mL to 500μg/mL,the concentration of standard substance and peak area were kept good linear relationship.The RSD% is between 0.08% and 6.81%,the coefficient of recovery reached 98.11%~102.25%.The limits of quantitation were from 52ng/mL to 53ng/mL.According to the result this method was better to detecting the ATP related compounds of fishery products.