对分离自生畜肉、生禽肉样品中的53株单核细胞增生李斯特菌的血清型及基因型进行了研究。53株单核细胞增生李斯特菌分属5个血清型,以1/2a,1/2b和1/2c血清型为主,分别占54.72%、20.75%和18.87%。以脉冲场凝胶电泳方法把53株单核细胞增生李斯特菌分成17个基因型(A-Q),其中Q型为主要基因型,占总数的22.64%。对2种分型方法进行综合分析能更好地了解单核细胞增生李斯特菌各菌株之间的遗传相关性及流行病学特征。

The fifty-three Listeria monocytogenes isolated from raw meat were characterized by serotyping and pulsed-field gel electrophoresis(PFGE).These strains distributed in 5 serotypes.The main serotypes were 1 /2a,1 / 2b and 1 /2c,the rates of which were 54.72%,20.75% and 18.87%,respectively.All tested strains were grouped into 17 PFGE types(A ～ Q) by genotyping using PFGE.Type Q was the most prevalent,accounting for 22.64% of all serotypes.These results indicate that the method based on PFGE in combination with serotypes analysis can be used to differentiate L.monocytogenes.