研究响应面法超声提取长白楤木中皂苷的工艺。在单因素实验的基础上,利用minitab15软件设计了响应面实验,研究乙醇浓度、料液比、超声提取时间和超声功率等因素对皂苷提取量的影响并确定最佳提取工艺参数。结果表明:长白楤木中皂苷类化合物的最佳超声提取工艺为乙醇体积分数为90%,料液比为1∶60(g∶mL),超声时间40 min,功率为250 W。预测模型所得提取量为45.14 mg/g,实际验证提取量为41.35 mg/g。

The ultrasonic-assisted extraction process of saponin from Aralia continentalis Kitag was studied.On the basis of single factor tests,response surface methodology was designed by minitab software 15,and the effects of ethanol concentration,ratio of solid to liquid,extraction time and extraction power on the extraction amount was studied.The result showed that the optimum extraction conditions were as follow: with 90% ethanol as the extraction solven and a ratio of solid to liquid of1 /60,extraction power at 250 W for 40 min.The predicted extraction amount was 45.14 mg/g,close to the practical extraction amount of 41.35 mg/g.