以1株谷氨酸高产的谷氨酸棒状杆菌(Corynebacterium glutamicum)A-26为出发菌株,先后采用亚硝基胍(NTG),紫外线进行诱变,并选用谷氨酰胺的2种结构类似物磺胺胍(SG)、甲硫氨酸亚砜(MSO)以及(NH4)2SO4筛选出具有三重抗性标记的谷氨酰胺高产菌株C.glutamicum N-U-6,其谷氨酰胺产量可达33.54 g/L,比出发菌株的产量(15.64 g/L)高出1.14倍。并检验了该诱变菌株具有较好的遗传稳定性。

Glutamine is the most abundant amino acid in the blood and has the ability to help preserve the intracellular pool of free amino acids in the body,which is a regulator of protein synthesis and correlates with nitrogen balance.Being the original strain,Corynebacterium glutamicum A-26 was mutagenized by NTG and the UV-radiation,then a mutant resistant to sulfaguanidine(SG),methionine sulfoxide(MSO) and(NH4) 2SO4 was obtained.It was named Corynebacterium glutamicu N-U-6.1.14 times glutamine(33.54 g/L) more than the original strain(15.64 g/L) was produced by the mutant.Its good hereditary stability was also tested.