采用4种蛋白酶(Neutrase酶、Alcalase酶、Protamex酶、Flavourzyme酶)对高底物浓度(24%)玉米黄粉进行双酶复合水解,研究复合水解对玉米蛋白酶解效率及玉米肽抗氧化活性的影响。结果表明,双酶复合水解可有效提高玉米蛋白的酶解效率,尤其是Flavourzyme酶与Alcalase酶复合,可使水解度提高26.85%,但酶解效率与抗氧化活性之间无直接对应关系。双酶复合水解对羟基自由基清除率影响较小,清除率均在90～100%范围内,与5%VC溶液的清除率相当;对DPPH自由基和超氧阴离子清除率影响较大,且两者的变化规律相反。

The proteases(Neutrase,Alcalase,Protamex,Flavourzyme) were used to perform multi-hydrolysis of high corn gluten meal concentration(24%),and the effects of double proteases on hydrolysis efficiency of corn pro-tein and antioxidant activities of corn peptides were studied.The result showed that the hydrolysis efficiency of double proteases was better than that of single protease,and Flavourzyme combining with Alcalase was the optimal design.In this condition,the degree of hydrolysis was increased 26.85%.The efficiency of hydrolysis was not positive correla-tion to the antioxidant activities of corn peptides.The scavenging rate of hydroxyl radical was 90% ～ 100%,equiva-lent to the effect of 5% Vitamin C solution.The multi-hydrolysis of double proteases had an obvious influence on the clearance rate of superoxide anion and DPPH radical of corn peptides,while the changes in the clearance rate of su-peroxide anion were contrary to that of DPPH radical.