对东北特色浆果红树莓花色苷进行了研究。提取后采用pH值示差法对所得花色苷粗提物进行定量分析,经测定红树莓花色苷粗提物中的花色苷含量为(7.548±0.03)mg/g(真空冻干粉),后采用大孔树脂柱层析的方法对所得花色苷粗品分离纯化,以AB-8作为层析介质,对红树莓花色苷粗提物的分离纯化工艺进行了优化,确定最佳分离条件为洗脱剂乙醇体积分数70%,pH值为1,洗脱剂体积为6倍柱体积,吸附解吸流速为2mL/min。通过HPLC-MS对花色苷种类及其结构进行分析鉴定,并最终鉴定得到9种花色苷,分别为矢车菊-3,5-双葡萄糖苷、矢车菊-3-双半乳糖苷、矢车菊-3-葡萄糖苷、矢车菊-3-芸香糖苷、矢车菊-3-阿拉伯糖苷、天竺葵-3-葡萄糖苷、芍药-3-葡萄糖苷、矢车菊和矢车菊-3-(6-O-乙酰基)-葡萄糖苷。

In this study,anthocyanins were extracted from wild red raspberries with 0.5% TFA in ethanol solution and were subjected to quantitative analysis.The total anthocyanin content of red raspberry crude extract,which was determined by pH differential method,was(7.548±0.03) mg/g of freeze-dried powder.Furthermore,the extracts were purified on a AB-8 macroporous resin column,the optimum elution reagent was 70% ethanol with TFA(pH 1),elution of ethanol was 6 times column volume at flow rate either on adsorption or desorption of 2 mL/min.Nine major anthocyanins of red raspberry were isolated.All of the peaks are identified by low-resolution electro spray mass spectrometry.HPLC-ESI/MS analysis results showed that the component of anthocyanins of the wild red raspberry fruit are cyanidin-3,5-diglucoside,cyanidin-3-digalactoside,cyanidin-3-glucoside,cyanidin-3-rutinoside,cyanidin,cyanidin-3-arabinoside,cyaniding-3-(6-O-acetyl)-glucoside pelargonidin-3-glucoside and peonidin-3-glucoside.