应用浸入式固相微萃取(DI-SPME)与气相色谱-质谱(GC-MS)联用技术,建立了快速检测白酒中较为常见的7种游离挥发性酚类化合物的方法。该方法在检测范围内有很好的线性关系(R2>0.99),酒样重复测定的相对标准偏差小于11%,检出限小于7μg/L,回收率大于85%。用该方法对不同香型成品白酒及浓香型原酒中游离挥发性酚类化合物进行测定,发现不同香型成品白酒中这些游离挥发性酚类化合物的种类、含量差异较大,其中浓香型成品白酒中酚总含量较高。在浓香型原酒中游离挥发性酚类化合物总含量变化与生产年份有一定的线性关系,生产年份越早的原酒中酚总含量越低。实验结果表明,该方法适用于白酒中游离挥发性酚类化合物的研究。

A rapid method for determining free volatile phenolic compounds in Chinese liquors(baijiu) was developed by direct immersion-solid phase microextraction(DI-SPME) coupled with gas chromatography-mass spectrometry(GC-MS).The method displayed good linearity(R2>0.99) over the concentration ranges explored in Chinese liquors.The analytical conditions enabled detection limits of free volatile phenolic compounds less than 7 μg/L.Precision(RSD<11%) and recovery rate were satisfactory in all cases.The content and kind of free volatile phenolic compounds in different aroma type liquors were significantly different,and the strong aroma type liquors had higher total content of phenolic compounds.There was a certain linear relationship between total content of phenolic compounds in strong aroma type liquors and production year of fresh distillates.The earlier production year of fresh distillates had lower total content of phenolic compounds in strong aroma type liquors.The results showed that the method provided a simple and quick technique to further investigate the free volatile phenolic compounds in Chinese liquors.