以转多功能纤维素酶基因(mfc)草菇子代中单孢分离获得的高产多功能纤维素酶菌株TVM186为研究对象,分析固态发酵对该工程菌株产多功能纤维素酶(MFC)的影响。通过单因素试验分析麸皮粉、大豆粉、香蕉皮粉等对MFC活性的影响,确定发酵主要影响因子为麸皮粉和大豆粉,适宜配比为质量比1∶1;利用均匀设计分析发酵条件对该菌株产MFC的影响,结果表明:草菇工程菌株TVM186固态发酵的适宜培养条件为:培养基起始pH值7.4,接种量6%,温度34℃,时间10 d,含水量64%,装料量18.5 g,其酶活力可达到木聚糖酶(Xylanase)1 427.38 U/g、内切-β-1,4-葡聚糖酶(CMCase)597.59 U/g。

TVM186 with high MFC activity was separated by monospore isolation from the transformants of Volvariella volvacea.Solid substrate fermentation was performed to investigate the optimum fermentation conditions for enzyme production of the transgenic V.volvacea.Several single factors on fermentation of strain TVM186 were studied in this research.The result showed that the optimum culture conditions of solid state fermentation were wheat bran power and soybean power with allocated proportion is 1:1.The optimum solid state fermentation conditions of TVM186 were initial pH 7.4,inoculation 6%,temperature 34℃,fermentation time 10 d,water ratio 64%,substrate 18.5 g using uniform design experiment.The highest Xylanase and CMCase activity were 1 427.38 U/g and 97.59 U/g respectively.