红枣经过浸泡、打浆、酶解、压滤、树脂吸附、甲酸洗脱后,采用150 nm纳滤膜在压差为0.5~1 MPa下过滤,结果表明:纳滤截留组分经过紫外扫描在波长258 nm处峰值与环磷酸腺苷(cAMP)标准品相同,而纳滤液组分(弃去液)在波长258 nm处无峰;又经过HPLC检测纳滤截留组分与环磷酸腺苷(cAMP)标准品出峰时间相同,而纳滤液组分(弃去液)在此时间处无峰。表明纳滤截留目标产物环磷酸腺苷(cAMP)效果明显,分离了大分子组分及小分子组分,纳滤后利于浓缩、纯化得到环磷酸腺苷(cAMP)成品。

Nanofiltration was used in the extraction of cAMP from Jujube.Jujube was first soaked,then through puree,enzyme hydrolysis,filtration,resin adsorption and formic acid elution.The results showed: Nanofiltration fraction have a peak at a wavelength of 258nm by UV which was the same as the cyclic adenosine monophosphate(cAMP) standard;the Nanofiltration discard part has no peak at 258nm;HPLC showed that Nanofiltration interception fraction has the same retention time as the cyclic adenosine monophosphate(cAMP) reference,while the Nanofiltration discard liquid do not have the peak at the same time point.This indicated that nanofiltration intercepted the target product of cyclic adenosine monophosphate(cAMP),successfully separated molecular,purified and concentrated cAMP.