在双有机溶剂体系中,用黑曲霉脂肪酶催化合成阿魏酸油醇酯,考察酶浓度、温度和底物摩尔比等因素对酯化反应的影响。结果表明:在异辛烷/丁酮体系中,当反应温度为60℃,阿魏酸和油醇的摩尔比为1∶8,即阿魏酸浓度为0.39 mg/mL和油醇浓度为4.3 mg/mL,脂肪酶浓度为0.2 g/mL时,转化率为97.6%;而在环己烷/丁酮体系中,当反应温度为60℃,阿魏酸和油醇的摩尔比为1∶8,即阿魏酸浓度为0.49 mg/mL和油醇浓度为5.37 mg/mL,脂肪酶浓度为0.25 g/mL时,转化率为91.0%。

In binary organic solvents system,the esterification between ferulic acid and oleyl alchol was investigated,catalyzed by the native Aspergillus niger lipase.The effective factors,such as lipase concentration,temperature and the molar ratio of ferulic acid to oleyl alchol on the esterification were studied.The results indicated that in the isooctane/2-butanone solvents system,under the reaction conditions of Aspergillus niger lipase concentration 0.2 g/mL,temperature 60 ℃,the molar ratio of ferulic acid to oleyl alchol 1∶ 8,substrate concentration of ferulic acid 0.39 mg/mL,and oleyl alcohol 4.3 mg/mL,the conversion rate of esterification was 97.6%.Under the optimal condition in the cyclohexane/2-butanone solvents system which was: Aspergillus niger lipase concentration 0.25 g/mL,temperature 60 ℃,the molar ratio of ferulic acid to oleyl alchol 1∶ 8,the substrate concentrations of ferulic acid at 0.49 mg/mL and oleyl alcohol at 5.37 mg/mL,the conversion rate of esterification was 91.0%.