以芭蕉芋糖浆为主要原料,利用琥珀酸放线杆菌(Actinobacillus succinogenes)CGMCC1593发酵生产丁二酸。单因素试验表明,培养基中总糖、玉米浆和磷酸盐对发酵有显著影响,利用响应面分析法(RSM)对培养基成分进行优化,并建立了各因素与丁二酸产量之间的数学模型。当各参数取值分别为总糖89.66 g/L,磷酸盐4.46g/L,玉米浆38.55 g/L,丁二酸最大估计值为62.92 g/L。优化后丁二酸产量比优化前提高了22.7%,与响应面预测极值基本相符。

Canna edulis Ker syrup was firstly used to product succinic acid by Actinobacillus succinogenes CGMCC1593.It was found that the total sugar,corn steep liquid and phosphate significantly improved the production of succinic acid.The Response Surface Methodology(RSM) based on a three-factor Box-Behnken design of experiments was used to build an analytical model and finally employed as a constraint in an obvious optimization process.The optimized parameters were total sugar of 89.66 g/L,corn steep liquid of 38.55 g/L,and phosphate of 4.46 g/L.Under those optimization conditions,it was predicted that the the highest concentation of succinic acid recached 62.92 g/L.After optimization,the succinic acid production increased by 22.7%,and the experimental data basically in line with the predicted values.