应用超滤-纳滤组合膜分离技术对泰乐菌素发酵液进行提纯和浓缩,以取代原有的板框过滤。对不同材料、截留分子量的超滤和纳滤膜的筛选结果表明,超滤膜宜采用PES-150聚醚砜膜,纳滤膜宜采用聚酰胺复合纳滤膜DL 2540。发酵液采用硅藻土预处理,且超滤过程恒容累积加水250L,能提高82%的膜通量,此时泰乐菌素的收率达97%。发酵液超滤液与原板框滤液的泰乐菌素组分纯度基本一致,但超滤液萃取效果更好,萃取收率提高了20%～26%。DL 2540纳滤膜对超滤液进行3.5倍浓缩,平均通量可达41.3 L/(m2.h),通过纳滤收率可达99%,纳滤浓缩液萃取无乳化现象,所得萃取液的色度也较低。

Instead of plat-and-frame filtration,separation processes with ultrafiltration(UF) and nanofiltration(NF) were introduced to purify and concentrate the tylosin broth.After comparing several membranes with different materials and molecular weight cut-off,PES-150 and DL2540 were found to be the optimum UF and NF membranes.With the pretreatment of tylosin fermentation by using diatomite and additive 250L de-ionized water,flux of UF would increase 82%.Meanwhile,yield coefficient of tylosin was almost 97%.Purities of each component of tylosin of UF and plat-and-frame filtrate were almost same.The yield coefficient of tylosin in extraction of UF filtrate increased 20%~26%.NF concentrated the UF filtrate with concentration multiples 3.5 and flux was 41.3 L/(m2·h).The yield coefficient of tylosin of NF was 99%.The color of extract phase of concentrated solution of NF,which without emulsification,was lighter than the ones without UF-NF.