利用超声与丙醇-硫酸铵双水相体系耦合对珍珠草多酚进行提取分离,基于单因素实验得到适宜条件:丙醇体积分数(A)60.0%、料液比(B)为1∶25.0(g∶mL)、超声时间(C)为30.0 min、(NH4)2SO4用量(D)为0.35g/mL。设计了四因素五水平的星点设计-效应面分析对提取条件进行优化,获得的最佳提取条件是:丙醇体积分数53.65%、料液比为1∶23.97(g∶mL)、超声时间为28.19 min、(NH4)2SO4用量为0.40 g/mL,珍珠草总多酚提取率E预测值为8.67%,与实验值E=8.72%(n=5)相符,优于单因素试验优化结果(8.61%,n=5)。

Ultrasound-assisted propyl alcohol ammonium sulfate aqueous two-phase extraction of polyphenols from Phyllanthus urinaria L.was investigated.The best condition for the single factor experiment was: propyl-alcohol concentration 60.0%,liquid/solid ratio B 25.0,extraction time C 30min.A Response surface methodology,with a five levels,four variables central composite designs was applied based on the single factor optimization experiment.The best condition was: propyl-alcohol concentration 53.65%,liquid/solid ratio 23.97,extraction time 28.19 min,and ammonium sulfate concentration 0.40 g/ml.An experimental extraction yield of 8.72 %(n=5)was achieved under the optimal extraction which was very close to the optimal prediction extraction yield(8.67 %),and was higher than that of single factor optimization experiment(8.61 %,n=5).