运用HPLC-DAD-ESI-MS技术建立了测定红树莓花色苷含量的方法,并确定了花色苷的成分组成。采用Zorbax SB-C18色谱柱(250 mm×4.6 mm,5μm),甲醇-5%甲酸水溶液为流动相,流速为1.0 mL/min,检测波长为520 nm,以矢车菊素-3-葡萄糖苷为对照,用外标法定量,并通过紫外扫描光谱信息和ESI+碎片离子信息对花色苷组成成分定性分析。结果表明:红树莓总花色苷含量为105.69 mg/100 g,其主要含有的4种花色苷成分为矢车菊素-3-槐糖苷、矢车菊素-3-槐糖-5-鼠李糖苷、矢车菊素-3-葡萄糖苷、矢车菊素-3-芸香糖苷,相对含量分别为22.05%、13.83%、33.74%和30.38%。

To establish a method to determine anthocyanin content and composition in red raspberry by HPLC-DAD-ESI-MS.Methods: The analysis was carried on Zorbax SB-C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-5% formic acid-water at the flow-rate of 1.0 mL/min,the detection wavelength was 520 nm and the scan wavelength was from 200 nm to 600 nm.Results and conclusion: The content of gross anthocyanins was 105.69 mg/100 g by HPLC calculated as cyanidin 3-glucoside equivalent.Four anthocyanins in red raspberry were identified by HPLC-DAD-ESI-MS,which are cyanidin 3-sophroside,cyanidin 3-sophroside 5-rhamnoside,cyanidin 3-glucoside and cyanidin 3-rutinoside.Their relative content was 22.05%,13.83%,33.74% and 30.38%,respectively.