对1株植物乳杆菌(Lactobacillus plantarum HSC 235)和1株费氏丙酸菌(Propionibacterium freudenreichiiHZP-35)生物转化果糖生成甘露醇能力进行了比较研究。在生长细胞、静息细胞以及无完整细胞粗酶提取物3种不同转化反应体系中,植物乳杆菌HSC 235和费氏丙酸菌HZP-35都表现出甘露醇脱氢酶活性,而生长细胞甘露醇产量和得率较高,分别为38 g/L和27%,静息细胞和粗酶提取物反应体系甘露醇的产量及得率则相关无几,表明甘露醇的转化过程与细胞生长紧密关联。在不同初始果糖浓度的试验中,较低果糖有利甘露醇得率的提高,50～150 g/L果糖浓度甘露醇得率稳定在27%左右,果糖浓度提高到200 g/L时则表现出对转化的抑制作用。植物乳杆菌可与费氏丙酸菌混合生长,但混菌发酵的实验结果表明,混合培养体系甘露醇得率比单菌种纯培养时甘露醇得率要低得多。植物乳杆菌甘露醇醇产量和得率要明显高于费氏丙酸菌。

D-Mannitol production from fructose by lactic acid bacteria(LAB,Lactobacillus plantarum HSC 235) and propionate acid bacteria(PAB,Propionibacterium freudenreichii HZP-35) was investigated.Fructose can be converted into mannitol and sorbitol.Three kinds of reaction systems of growing cells,resting cells,and free cell extract were compared in mannitol formation using fructose as substrate.The results showed that the growing cells reaction process gave out a higher mannitol yield at 38 g/L,the Yp/s at 27%,while the last two reaction processes had a similar low mannitol yield.This suggested that mannitol formation was coincided with the cell growth of LAB and PAB.Initial fructose concentration hadn't appear effect on mannitol formation between 50-150 g/L where the mannitol Yp/s was at 27%,but high up to 200 g/L fructose had a inhibition on the conversion process.Although LAB and PAB can grow together as in many food,but the mixed cultivation of the two bacteria would not produce more mannitol compared with any single pure strain fermentation.In the ability of mannitol production,LAB showed a high level to PAB.