以总三萜的洗脱效果为考察指标,研究了大孔树脂分离纯化的吸附性能及洗脱参数。结果表明,XAD-16大孔树脂适宜樟芝胞外总三萜的富集分离,其再生能力良好,吸附过程符合Langmuir方程。同时获得了XAD-16大孔树脂对三萜类化合物的动态分离条件:上样发酵液的pH为6.0,吸附流速为2 mL/min,解吸附溶剂为乙醇。溶液中三萜类化合物的初始浓度为0.97 mg/mL,获得了纯度为260.54 mg/g的总三萜样品。

Based on desorption effect on total triterpenoids in culture broth of Antrodia camphorata,the adsorption capability and desorption parameters of macroporous resin were studied.The best macroporous resin,XAD-16,has good regeneration ability and the adsorption process is in accordance with Langmuir equation.The optimal separation conditions were as follows: culture broth with pH of 6.0,flow rate of 2 mL/min,100% Ethanol as desorption solution.After separation by macroporous resin,total triterpenoids sample with the purity of 260.54 mg/g was obtained from culture broth of Antrodia camphorata.