研究AB-8大孔树脂法去除车前草粗多糖中蛋白质的适宜条件。采用动态吸附和解析实验对树脂纯化工艺进行优化。结果表明适宜工艺条件为:上样液浓度40mg/mL,上样流速0.5 mL/min,上样液pH值7.0;以蒸馏水为洗脱剂,洗脱速度2 mL/min,洗脱体积2.5BV(1BV=20 mL)。纯化后AB-8大孔吸附树脂对车前草粗多糖中的蛋白具有较高的去除效果,蛋白去除率为84.83%,多糖保留率为88.32%。

The article studied the optimal conditions of removing protein from polysaccharide of Plantago asiatica L.with AB-8 macroporous adsorption resin.The purification conditions were optimized by dynamic adsorption and desorption.The results showed that the optimum conditions were as follows:the concentration of solution was 40 mg/mL with pH of 7.0,the sample loading flow rate was 0.5 mL/min.The elution solvent was 2.5BV distilled water at flow rate of 2mL/min.AB-8 macroporous adsorption resin had the best effect,the protein removing rate was 84.83% and polysaccharide retained rate was 88.32%.