在5 L发酵罐上,分别考察了亚硒酸钠添加时间和L-蛋氨酸对富硒产朊假丝酵母制备过程中的细胞量、谷胱甘肽合成、胞内谷胱甘肽含量以及硒有机转化率的影响。以高性能(高有机硒含量、高GSH含量)富硒酵母的制备为目标,得到一种比较有效的发酵培养策略,即发酵初期(0 h)添加L-蛋氨酸以及葡萄糖耗尽时(15h)添加亚硒酸钠,最终硒有机转化率达到87.5%。

The effects of Na2SeO3 addition time and L-methione on the biomass,glutathione biosynthesis,intracellular glutathione content and selenium organic transformation rate during selenium-enriched Candida utilis preparation in a 5 L stirred fermentor were investigated.An effective culture strategy which involved L-methione addition at the beginning of cultivation(0 h) and Na2SeO3 addition after glucose consumption(15 h) was obtained with the aim of high organic selenium content and high intracellular glutathione content within the selenium-enriched C.utilis.Using this strategy,the selenium organic biotransformation rate can reach 87.5%.