以玉米内源基因IVR、外源抗除草剂基因(BAR、PAT)、抗虫基因Cry1Ab、筛选基因NPTII、CaMV35S启动子和NOS终止子为检测的目的片段,分别设计了7对引物,通过研究较佳引物终浓度配比和退火温度,建立了玉米转基因成分七重PCR检测体系。结果表明,建立的七重PCR体系用于同时检测内源基因和转基因成分是可行的,检测方法效率高、稳定性好。

To establish Seven-plex PCR detection system for detecting transgenic components in genetically modified Maize,endogenous gene IVR,exogenous herbicide gene BAR and PAT,exogenous insect resistant gene Cry1Ab,screening gene NPTII,CaMV35S promoter and Nos terminator were chosen for the targeted fragment.7 pairs of primers are designed and the PCR conditions for the multiplex PCR analysis were optimized according to the primer concentration and annealing temperature.The results show that Seven-Plex PCR system can detect endogenous gene and transgenic components with high efficiency and good stability.