采用甲醇解和硅烷衍生化,用气相色谱法同时测定样品中的中性糖、糖醛酸、N-乙酰氨基糖和唾液酸,取得了很好的效果。3种标准单糖混合物(包含阿拉伯糖、鼠李糖、岩藻糖、木糖、甘露糖、半乳糖、葡萄糖、葡萄糖醛酸、半乳糖醛酸、N-乙酰半乳糖、N-乙酰葡萄糖、5-乙酰唾液酸)和2种多糖样品(豆腐渣多糖DFP、鸡腿菇多糖F32)用1 mol/L盐酸甲醇在85℃反应18～24 h,碳酸银中和,加入乙酸酐室温暗处反应24 h,使氨基糖重新引入N-乙酰基,除银盐,干燥,加入硅烷化试剂[V(吡啶)∶V(六甲基二硅氨烷)∶V(三甲基氯硅烷)=5∶1∶1),室温放置30 min,最后用气相色谱进行分析。

Neutral sugar,uronic acid,amino sugar and sialic acid were effectively determined simultaneously by gas chromatography after methanolysis and trimethylsilylation.Three standard monosaccharide mixtures(including Ara,Rha,Fuc,Xyl,Man,Gal,Glc,GlcA,GalA,GalNAc,GlcNAc,Neu5Ac) and two polysaccharide samples(okara polysaccharide DFP,Coprinus comatus polysaccharide F32) were methanolysis by 1 mol/L HCl/methanol at 85 ℃ for 18~24 h,neutralized with Ag2CO3 and re-N-acetylated by adding acetic anhydride for 24 h in the dark.After removing the silver salts,the dried residue was trimethylsilylated by adding silylation reagent(pyridine/hexamethyldisilazane/chlorotrimethylsilane = 5∶ 1∶ 1) for 30 min at room temperature.Then the final products were analyzed by gas chromatography.This method needs fewer amount of sample,and has higher sensitivity and resolution.It can simultaneous detect kinds of monosaccharides.It will have a great potential for application.