以富含纤维素的豆渣为原料,采用酸预处理粉碎后的豆渣,预处理条件为3 mol/L的HCl,水解温度100℃,水解时间120 min,HCl溶液添加量与原料比值为50∶1(mL∶g)。以酸预处理干燥后得到的样品作为纤维素酶水解的原料,通过单因素和正交实验,获得制备纳米纤维素的最佳酶解条件:酶用量3 000U/g,pH5.0,温度55℃,时间6 h,液料比20 mL/g。通过扫描电镜和透射电镜检测,制备出的纳米豆渣纤维素呈微球状,粒度为15～50nm。

Nano-sized cellulose has excellent characters.The paper used soybean dregs which are rich in cellulose as raw materials.The grinding soybean dregs were pretreated by hydrochloric acid.The pretreatment conditions are 3 mol/L HCl in concentration,hydrolyzation temperature 100 ℃,hydrolyzation time 120 min,acid water to material ratio 50∶ 1.The soybean-dreg cellulose pretreated with HCl and dried was hydrolyzed with cellulase.The optimum preparation technology of nano-sized soybean-dreg cellulose was investigated by single factor and orthogonal experiments: cellulase dosage 3 000U/g,pH 5.0,hydrolyzation temperature 55 ℃,hydrolyzation time 6 h,water to material ratio 20 mL/g.The size of nanocrystalline cellulose with globosity appearance was 15~50 nm through scanning electron microscope and transmission electron microscope.