评价了双层平板(DLAP)计数法对海产食品中副溶血性弧菌的定量分析效果。通过在混合菌悬液和鱼、虾、贝等生物体中人为模拟染菌,并进行冷冻受伤减菌处理,采用DLAP计数法对副溶血性弧菌处理前后的菌量变化进行分析,同时采用MPN法、BCVM平板计数法和TCBS平板计数法3种方法作为对照,运用SPSS软件分析4种方法测定结果的显著性差异(P<0.05)。试验结果表明,在理想的控制条件下,4种方法对不同基质条件中副溶血性弧菌的测定结果不存在明显差异;在菌体活力不强或受伤状态下,DLAP计数法对该菌的测定结果与MPN法在同一水平上且缩短了5d的检验周期,其准确性优于BCVM平板计数法和TCBS平板计数法,具有省时、准确的特点,适合广普应用。

This study was about the evaluation of a double layer plate method to detect Vibrio parahaemolyticus(V.p) in seafood.Throughout adding a certain amount of V.p in mixed cultures and seafood samples and applying frozen treatments to make V.p injured,four methods(DLAP plate,MPN method,BCVM plate and TCBS plate) were adopted to detect V.p in every experimental sample,the results of which were analyzed by SPSS software(P<0.05).The results demonstrated that the four methods were not significantly different from each other in different samples under the ideal experimental conditions.But if V.p was inactive or injured,the results of DLAP plate for detecting V.p,which could reduce at least 5 day detecting period compared to MPN method,were as effective as MPN and more accurate than BCVM plate or TCBS plate.Because of its time-saving and exactness,the DLAP plate offers a broad application for quick detecting and screening of V.p in seafood.