以淋巴细胞增殖率为主要指标,在单因素的基础上,采用响应面法优化了超声提取羊胎盘中水溶性活性蛋白质的工艺,建立了二次多项模型,研究超声功率、固液比、超声时间3个因素及其交互作用对提取工艺的影响。试验结果表明,对蛋白质提取率的影响次序为超声功率>固液比>超声时间;最优工艺参数是:超声功率为300W,固液比为1:30,超声时间25min,淋巴细胞增殖率达53.17%。

In order to timely extract protein compounds from Sheep Placenta,investigation of ultrasonic -assisted extraction was carried.The entire experiment was carefully designed and optimized with response surface method (RSM).A second quadratic equation for protein extraction was finally obtained.Factors,such as extraction temperature, solid-liquid ratio,extraction time and their corresponding relationships were investigated.Results showed that the effectiveness order of four factors is as follows:solid-liquid ratio > extraction time > extraction temperature.The optimized extraction conditions were solid-liquid ratio > extraction time > extraction temperature.Under the optimal conditions,the extraction rate of protein from Sheep Placenta reached to 53.17%.