利用响应面法对猫须草总黄酮的提取工艺进行优化。在单因素试验基础上,选择温度、乙醇浓度和液料比为自变量,总黄酮提取率为响应值,利用响应面分析法,研究各自变量交互作用及其对总黄酮得率的影响,模拟得到二次多项式回归方程的预测模型。猫须草总黄酮的提取工艺为温度70℃、乙醇浓度54%、液料比20:1(mL:g)。在最优工艺条件下总黄酮得率为7.6%。

Objective:To maximize flavonid extraction yield,optimization of the extraction of total flavonoid from clerodendranthus spicatus under ultrasound assistance was achieved by response methodology.Methods:On the basis of single -factor experiments,Box-Behnken design(BBD) involving three variables at three levels was utilized to set up a quadratic polynomial regression equation.Extraction temperature,ethanol concentration and liquid/material ration, and pair-wise interactive effects of above three variables on their function were investigated by response surface analysis.Results;The optimum condition were;30min ultrasound treatment at 100%power,ethanol concentration 54%,liquid and solid ratio was 20:1,extracting temperature 70℃.Conclusions:Under these optimum conditions, the yield of total flavonoid was 7.6%.