采用化学诱变剂亚硝基胍(NTG)对纳他霉素生产菌株Streptomyces natalansis PM2-5孢子进行诱变处理,再根据浅蓝霉素对大环内酯合成途径中多聚乙酰合成酶(Polyketide synthase,PKS)的抑制作用机制,以浅蓝霉素作为筛选因子,选育出耐浅蓝霉素的高产突变菌株,获得高产突变菌株PM4-18,其生产能力是出发菌株的1.67倍,菌株遗传性能稳定。
Spores of Natamycin producing strain S.natalansis PM2 -5 were treated with nitrosoguanidine,and then were selected with cerulomycin which could inhibit Polyketide synthase of the macro - lactone and the growth of Natamycin that produces strain and its product synthesis.Anti-cerulomycin Natamycin high - producing strain PM4 - 18 was obtained.Its productivity was improved 1.67 times and could be inherited stably.
