研究XAD-7HP大孔树脂分离纯化喀什小檗花色苷的纯化工艺条件。通过树脂筛选和单因素实验确定适宜工艺参数:吸附时间是4h,体积分数60%乙醇溶液为洗脱液,解析时间100min,上样液pH值2.0,吸附温度为30℃,上样质量浓度为750mg/L,洗脱流速为1.0mL/min,。该工艺生产的花色苷产品为紫黑色粉末,色价和花色苷含量比纯化前分别提高了2.21倍和3.15倍。

The condition of purification of anthocyanins from Berberis kaschgarica by XAD-7HP macroporous resins was studied.The tests of screened resins and single-factor were used to determin the optimal technological parameters: 4 hours of adsorption time,60%ethanol as eluant,desorption time 100 min,pH 2.0,temperature 30℃.The concentration of anthocyanins is 750 mg /L and flow rate is 1.0 mL/min.Under the above optimum conditions,content of anthocyanins and colour value increased 3.15 and 2.21 times respectively in dark purple powder final product.