以"沙家浜"粗绿茶为原料制取茶多糖,并对茶多糖进行脱蛋白和脱色纯化研究。结果表明:聚酰胺吸附柱层析法作为茶多糖纯化(脱蛋白、脱色)的最佳方法,其脱色的适宜工艺条件为,聚酰胺用量为粗多糖质量的6倍,聚酰胺柱用1.5倍柱体积的去离子水以1.5mL/min的流速进行洗脱,在此工艺条件下,蛋白质脱除率达到95.8%。

Crude green tea chang shu shajiabang was used as raw material to prepare tea polysaccharides.It then were purified by deproteinization and decolorization.The result showed that polyamide adsorption was the best purification (deproteinization and decolorization ) methods.The optimum decolorization technology was:The content of polyamide was 6 times of crude polysaccharides,and the polyamide column was eluted with 1.5 column volumes of deionized water at flow rate of 1.5mL/min.Under this technology,the protein removing rate was 95.8%.