研究了10、15、20、25、30和35kV/cm的高强度脉冲电场对大肠杆菌的杀菌效果及其对细胞膜结构的影响。结果表明,当电场强度超过20kV/cm,处理时间为200μs时,大肠杆菌细菌数量显著降低,同时采用流式细胞仪分选碘化丙啶(PI)标记细胞结果表明,电场强度超过20kV/cm,细胞膜受到破坏,通透性增强,PI标记细胞比例增加。

The effects of high - intensity pulsed electric field on inactivation and cell membrane of E.coli were investigated.The results showed that when the applied electric intensity was higher than 20 kV/cm for 200μs,the number of survivable E.coli significantly decreased.The flow cytometry analysis of E.coli cells stained with PI showed high - intensity pulsed electric field could destroy the cell membrane and increase the permeability.When the applied electric intensity was higher than 20 kV/cm for 200μs,the PI stained cells percentage by flow cytometry sharply increases.