以万年蒿为原料,研究超声波提取万年蒿总黄酮的最佳工艺。通过单因素试验研究乙醇体积分数、料液比、超声波功率及超声时间对总黄酮提取量的影响。以此为基础,采用L9(34)正交试验设计,优化超声波提取万年蒿总黄酮工艺条件;并将其与传统的热回流法和浸提法进行了比较。超声波提取万年蒿总黄酮的最佳提取工艺参数为:乙醇体积分数60%,料液比1∶20(g:mL),超声波功率350 W,超声时间60 min。在此工艺条件下,万年蒿总黄酮提取量为69.55 mg/g。

Artemisia sacrorum Ledeb.was extracted by ultrasound and the processing was optimized.The influence of four extraction variables on extraction yields of total flavonoids was investigated,including ethanol concentration,the ratio of material to solvent,ultrasonic power and time.Based on the results of our investigations,orthogonal test design L9(34)was used to optimize the extraction yields.In addition,heat reflux and maceration were also employed to compare with ultrasonic extraction processes.The results indicated that the optimum ultrasonic extraction conditions were as follows: ethanol concentration 60%(v/v),the ratio of material to solvent 1∶ 20(w/v),ultrasonic power 350 W,time 60 min.Under the above conditions,the total ?avonoids yields could arrive 69.55 mg·g-1.Meanwhile,the optimized ultrasonic extraction processes was stable and feasible.In addition,it had several remarkable advantages,including time saving,high efficiency and easier-operation.