以八成熟的海南蒲桃果实粉末为试材,采用正丁醇-盐酸法显色、测定提取液的吸光度,研究了八成熟海南蒲桃果实原花青素的提取工艺。经单因子实验及正交实验结果表明,以体积分数60%丙酮溶液(pH=3)为浸提液、料液比1∶9(g:mL)、温度60℃及时间2 h为最适工艺参数,经2次浸提原花青素的提取率为91.52%。八成熟的海南蒲桃果实原花青素含量5.70%,为充分成熟果实的127.23%。

S.cumini fruit at eighty percent ripe stage was used as material for this research.The extraction of proanthocyanidins from the fruits was studied by butanol-hydrochloric acid technology.The yield was by measuring the absorbance of the product.According to single factors tests and orthogonal optimization,the results showed that the optimal conditions were: concentration of acetone were 60%(pH=3),the ratio of extraction solution to sample was 9,the extraction temperature was 60℃ and the extraction time was 2h.The proanthocyanidins yield was 91.52% after being extracted 2 times.The proanthocyanidins content in the S.cumini fruit at eight percent ripe stage was 5.96%,which was 133.04% times of fully ripen fruits.