采用MTT法测定细胞增殖、流式细胞术分析细胞周期及细胞存活率,对玉米低聚肽的促HL-7702人肝细胞增殖作用进行了研究。结果显示:玉米低聚肽在1～10 g/L内,可促进肝细胞生长,其促进作用与肽浓度呈正相关;给予肝细胞玉米低聚肽24 h后,细胞周期中S期及G2/M期细胞的比例上升;并且在此浓度范围内,玉米低聚肽对肝细胞存活率及形态影响较小。研究表明,玉米低聚肽有促进HL-7702人肝细胞增殖及DNA合成的作用,可显著提高肝细胞活力,提示其在保肝护肝中可能具有一定作用。

The proliferation-promoting effects of corn oligopeptides(COP) on HL-7702 cells in vitro were studied by MTT assay and flow cytometry(FCM).Results indicated that COP could stimulate the growth of hepatocyte,and the promotion increased in a concentration-dependent manner over the range of 1~10 g/L;the proportions of cells in S and G2/M phases of cell cycle were increased at 24h after the addition of COP;and in this concentration range,the influence of COP on cell viability and morphology were not significant.COP could enhance human hepatocyte line HL-7702 proliferation and DNA synthesis,and also can improve the vitality of hepatocyte.The study suggested that COP could have certain effect on liver protection.