在研究亚硝酸钠与费氏弧菌发光抑制率存在相关性的基础上,建立了评价亚硝酸钠生物毒性的方法,并将其应用于果汁中亚硝酸钠综合毒性的检验。结果显示,发光强度抑制率与亚硝酸钠溶液质量浓度呈正相关,相关系数在0.953 3～0.957 3,最佳检测平衡时间为15 min,误差限范围在0.615 0～2.346 6;发光强度抑制率与果汁中亚硝酸钠质量浓度同样呈正相关,相关系数在0.955 5～0.997 0,最佳检测平衡时间为15 min,误差限范围在0.085 2～2.448 3。

A method built on the basis of the toxicity effect of sodium nitrite on luminescent bacterium Vibrio fisheri was developed for determining the toxicity and content of sodium nitrite.Results showed that there was a positive correlation between inhibitory rate of sodium nitrite against luminescent intensity of Vibrio fisheri and sodium nitrite concentration in aqueous solution,with a correlation coefficient ranging within 0.9533-0.9573.The optimal balance time was 15min.Error limit range is 0.6150-2.3466.Inhibitory rate of sodium nitrite against luminescent intensity of Vibrio fisheri was also positively correlated with positive concentration in fruit juice,and the correlation coefficient was between 0.9555 and 0.9970.The optimal balance time was 15min.Error limit range is 0.0852-2.4483.For the establishment of luminescent bacteria toxicity assay lays a foundation.