通过比较11种大孔吸附树脂对胡芦巴黄酮类化合物的静态吸附与解吸性能,筛选出DM130型大孔吸附树脂用于分离纯化胡芦巴种子中的黄酮类化合物。采用单因素方法分析该树脂富集纯化胡芦巴总黄酮的适宜工艺条件,确定优化的工艺条件为:上样量为3.64 mg黄酮/g树脂,上样液pH值5.0,吸附时间2 h,体积分数70%乙醇洗脱,洗脱速率2 mL/min,洗脱体积为150 mL,总黄酮回收率为85.05%,提取物中黄酮含量由7.8%提高到26.5%。

Eleven types of macroporous resins were applied to study static adsorption and desorption performances towards Trigonella foenum-graecum L.seeds flavonoids,and DM130 was the selected for the separation and purification of the crude flavonoids from Trigonella foenum-graecum L.prepared with ultrasonic-assisted extraction.Single factor experiments showed that the optimum purification procedure of total flavonoids from Trigonella foenum-graecum L.were determined as follows: sample loading amount 3.64 mg total flavonoids /g macroporous adsorption resin at pH 5.0,adsorption duration 2 h,flow rate 2.0 mL/min,70% ethanol,desorption volume 3BV.Under the above optimized purification process,the recovery of flavonoids was 85.05% and the purity increased from 7.8% to 26.5%.