研究了响应面法优化超声辅助提取酸枣叶中总黄酮工艺。在单因素实验的基础上,选择液料比、乙醇浓度、超声功率、超声时间四因素,利用Design-Expert 7.1.6软件Box-Benhnken中心组合试验和响应面分析法,研究了各自变量交互作用对酸枣叶总黄酮得率的影响,模拟得到二次多项式回归方程的预测模型。结果表明:酸枣叶总黄酮的适宜超声提取工艺为,液料比42.00∶1(mL∶g)、乙醇体积分数60.10%、超声功率240.00 W、超声提取时间36.50 min。总黄酮得率实测值为5.382%,与预测值5.407%相符良好。

The ultrasonic-assisted extraction process of total flavonoids from the leaves of Ziziphus jujuba var.spinosa was studied.Based on the experiment of single factor,the mathematical regression model was established about the dependent variable(extraction yield of total flavonoids) and independent variables(ratio of liquid to solid,ethanol concentration,extraction power and extraction tine) through Box-Benhnken center composite design and response surface methodology.The optimum extraction conditions were as follow∶ratio of liquid to solid of 42.00∶1,ethanol concentration of 60.10%,extraction power of 240 W and extraction time of 36.50 min.Under these conditions,the practical yield of total flavonoids reached to 5.382%,which was consistent with the predicated yield of total flavonoids of 5.407%.