以蚕豆(启豆2号)为原料,研究了低氧联合NaCl胁迫下培养条件对γ-氨基丁酸(GABA)富集的影响。结果显示:非胁迫培养时间、培养pH和胁迫培养时间显著影响发芽蚕豆GABA积累。蚕豆发芽富集GABA最佳培养条件是非胁迫培养1.5 d、培养液pH 3.5和低氧联合NaCl胁迫4 d,在此条件下其GABA含量可达1.06mg/g DW,为原料蚕豆的7.57倍。

The culture condition of γ-aminobutyric acid accumulation in germinating fava bean(Qi Bean 2) seeds under hypoxia combined with NaCl was investigated.The results showed that non-stress culture time,pH and stress time impacted the accumulation of GABA significantly.The optimum condition was culturing for 1.5 days,pH 3.5 and stress for 4 days.The GABA content was 1.06 mg/g DW and was 7.57-fold of that in un-germinated fava bean.