花生中富含辅酶Q10,以高效液相色谱法测量的辅酶Q10含量为指标,系统研究了利用超声波细胞破碎仪从花生中提取辅酶Q10时不同提取条件对其含量的影响。结果表明:4个因素对提取率影响大小的顺序为:提取功率>提取时间>单次辐射时间>料液比,其中提取功率和提取时间对花生中辅酶Q10的提取率有极显著影响。最佳工艺参数为:提取功率400 W,提取时间15 min,单次辐射时间为6.0 s/5.0,料液比为1∶6,在此条件下花生中辅酶Q10的提取率为92.4μg/g。

Peanut contains coenzyme Q10,the content of Q10 was detected by HPLC.The effect of different conditions by using ultrasonic cell-break method for extraction was systematically studied.The results showed that the size order of influence by four factors on the extraction rate was: extraction power > extraction time > duration of radiation each time > ratio of material to liquid.The power extraction and extraction time had significant effect on the extraction rate of coenzyme Q10.The optimal condition for coenzyme Q10 extraction was as follows: 400W,extraction time: 15min,duration of radiation: 6.0s/5,ratio of solid to liquid : 1∶ 6.Under this condition,the coenzyme Q10 extraction rate was 92.4ug/g.