通过单因素试验分析了乙醇体积分数、液料比、提取温度和提取时间对橙皮苷得率的影响,在此基础上选择4因素3水平的Box-Behnken中心组合试验设计,采用响应面法对橘皮中橙皮苷的提取工艺进行了优化。结果表明:最佳提取工艺条件为溶剂中乙醇体积分数71﹪,液料比22∶1 mL/g,提取温度70℃,提取时间2.5 h。经试验验证,在此条件下,得率达4.82%。对橙皮苷提取液的抗氧化研究表明,橙皮苷对羟自由基有一定的清除作用。

Four factors including ethanol concentration,solvent to material ratio,extraction temperature and extraction time were studies on the yield of hesperidin from the citrus peel by using single factor analysis method.Then a four factor,three-level Box-Behnken central composite design(CCD) coupled with response surface analysis(RSM) was applied in optimization of the extraction.It was found that the optimum extraction conditions were: ethanol concentration 71%,solvent to material ratio 22∶ 1(mL/g),extraction temperature 70℃ and extraction time 2.5 h.Under the optimized extraction conditions,hesperidin extraction yield was 4.82%.The research on anti-oxidation properties showed that the extracted hesperidin had scavenging activity on free hydroxyl radical.