对溪黄草中的多酚提取物的抗氧化性进行了研究。通过DPPH自由基清除实验、过氧化氢清除实验、还原力实验、抑制β-胡萝卜素褪色实验以及抑制亚油酸过氧化实验,分别研究了溪黄草多酚与抗坏血的抗氧化能力。结果表明:溪黄草多酚提取物具有良好的抗氧化能力,其清除DPPH自由基、清除过氧化氢、抑制亚油酸过氧化的IC50值分别为5.00、42.51与66.58μg/mL,高于抗坏血酸相应的IC50值,即5.99、56.74与94.83μg/mL。表明在试验范围内,溪黄草多酚的抗氧化活性强于抗坏血酸。

Antioxidant activities of polyphenol extracted from Rabdosia serra(Maxim.) Hara were studied.DPPH radical scavenging assay,H2O2 scavenging assay,reducing power assay,β-carotene bleaching assay and inhibition of linoleic acid peroxidation assay were conducted.The antioxidant activities of polyphenol from Rabdosia serra(Maxim.) Hara was compared with VC.Results showed that the antioxidant activities of polyphenol from Rabdosia serra(Maxim.) Hara were better than that of VC.The IC50 of polyphenol from Rabdosia serra(Maxim.) Hara on DPPH radical scavenging assay,H2O2 scavenging assay,β-carotene bleaching assay and inhibition of linoleic acid peroxidation assay were 5.00 μg/mL,42.51 μg/mL and 66.58 μg/mL respectively;the above assay on VC were 5.99 μg/mL,56.74 μg/mL and 94.83 μg/mL respectively.These results indicated that the antioxidant activities of polyphenol from Rabdosia serra(Maxim.) Hara were better than that of VC.