研究了重庆市涪陵区所产龙眼混合果蔬汁乳酸菌发酵饮料的工艺条件,以优质龙眼、胡萝卜、番茄汁为原料,用德氏乳杆菌保加利亚亚种和嗜热链球菌为菌种,进行菌种活化和驯化。确定了菌种、中间种子扩大液、发酵液及发酵饮料口感稳定性的工艺配方。结果显示,用德氏乳杆菌保加利亚亚种和嗜热链球菌按1∶1比例作为菌种;种子扩大液的最佳配方为龙眼、胡萝卜、番茄混合汁配比3∶6∶1,3%葡萄糖,4%脱脂乳,2%接种量;发酵液配方:龙眼、胡萝卜、番茄混合汁配比3.5∶5∶1.5,发酵温度37℃,发酵时间24 h,接种量4%;发酵饮料调配配方:发酵原液中添加蔗糖6%,柠檬酸0.04%,CMC-Na 0.1%,黄原胶0.01%。

The development of production technology of lactic acid bacteria beverage mixed with Longyan from Fuling District,Chongqing and other fruits and vegetables juiceswas studied.Methods: activation and domestication were conducted using the juices of longyan,carrot and tomato with high quality as raw material,using Bulgarian subsp of Delbrueckii and Streptococcus thermophilus as inoculation strains.The optimum formulation for the intermediate seed expanding liquid,fermentation and fermentation beverage taste stability was determined via four factors and three levels orthogonal experiment.Results : Bulgarian subsp of delbrueckii and Streptococcus thermophilus with 1∶ 1 ratio were used as inoculation strains.The best formula of seeds expanding fluid was as follows: the ratio of lonyan to carrot to tomato juice was 3∶ 6∶ 1,3% glucose,4% skim milk,2% inoculum.Fermentation liquid formula was as follow: ratio of longyan to carrot to tomato juice was 3.5∶ 5∶ 1.5.Fermentation was carried out at 37 ℃ for 24 h and inoculum volume was 4%.The optimum formula of fermented beverage of fermented liquor was as follows: 6% of sugar,0.04% of citric acid,0.1% of CMC-Na and 0.01% yellow former glue.