从原料奶中分离富集出芽孢,应用紫外分光光度法对芽孢中的2,6-吡啶二羧酸进行检测,在385 nm至265 nm之间进行紫外扫描,求得一阶导数光谱,根据283 nm至276 nm的最小值与最大值之差求得2,6-吡啶二羧酸的含量,通过与芽孢的平板计数相比较,该方法与平板计数法呈明确的线性关系,可以对原料奶中的芽孢水平进行评估。

This article first isolated spores from raw milk,and then applied the method of ultraviolet spectrophotometry to detect the contains of 2,6-dipicolinic acid,scaned between 385 nm to 265 nm and the first order derivative spectrum was obtained.Calculated the content of 2,6-dipicolinic acid by diffrence between the minimum and the maximum from 283 nm to 276 nm.Compared with the plate count,it showed a clear linear relationship between them,so the level of the spores in the raw milk can be assessed by the method of ultraviolet spectrophotometry.