建立了超高效液相色谱-串联质谱/质谱法高通量快速测定调料中吗啡、可待因、蒂巴因、罂粟碱、那可丁含量。样品通过乙腈-盐酸溶液提取,MCX固相萃取柱净化预处理后用超高效液相色谱-串联质谱/质谱仪进行检测。流动相为0.1%的乙酸-乙腈溶液+10 mmol/L乙酸铵溶液,梯度洗脱,流速为0.2 mL/min,柱温40℃。该方法对调料中罂粟碱、那可丁最低检出限分别为0.8、1.0μg/kg,吗啡、可待因和蒂巴因的最低检出限为50μg/kg,8 min就可以全部检出。

An ultra performance liquid chromatography-tandem mass spectrometry method was developed for the separation and determination of alkaloids in papaveris content in seasonings.After the hydrochloric acid extraction and MCX purification,the samples were injected into the liquid chromatography-tandem mass spectrometry.The chromatographic separation was performed at 40℃ in the gradient elution mode using a mobile phase composed of a mixture of 0.1% acetic acid-acetonitrile and 10 mmol/L ammonium acetate at the flow of 0.2 mL/min.In this method,five kinds of alkaloids can be detected within eight minutes.The minimum detection limit of Papaverine and Narcotine were 0.8 μg/kg and 1.0 μg/kg,the minimum detection limit of Morphine,Codeine and Thebaine were 50 μg/kg.This method was simple and high sensitive and precise.It has a potential for practical applications in alkaloids determination.