以β-CD的乙醇溶液为介质,用微波辅助提取山楂中的熊果酸,并用紫外分光光度法测定山楂中熊果酸的得率。在单因素实验的基础上,采用正交试验。研究表明适宜的提取工艺条件为:乙醇体积分数为80%,微波功率50%,微波提取时间90 s,料液比(g∶mL)1∶25,β-CD的用量m(山楂)∶m(β-CD)为1∶1。正交试验优化结果为:乙醇体积分数80%,微波时间为100 s,微波功率为60%,m(β-CD):m(山楂)为1∶1。在优化提取条件下,山楂中的熊果酸得率为:3.374mg/g。β-CD的加入有效的提高了熊果酸的得率。

The ursolic acid from Crataegus was extracted by β-CD-ethanol solution assisted with microwave.The yield of the ursolic acid was determined by UV-spectrophotometry.The optimum extraction conditions were investigated through orthogonal test according to single factor experiment.The optimum extraction conditions were: ethanol concentration 80%,microwave power 60%,extraction time 100 s,solid to liquid ratio of 1∶ 25 and the amount of crataegus: m(β-CD) 1∶ 1.Under the optimum conditions,the yield of ursolic acid was increased to 3.374 mg/g with the presence of β-CD.