研究铜藻提取液通过径向流色谱快速脱除蛋白质的工艺参数,以蛋白质脱出率和多糖保留率为考查指标,考察进样浓度、进样量、进样速度以及洗脱速度参数的影响。结果表明:当上样流速5 mL/min,洗脱速度40mL/min,上样量100 mL,上样浓度5mg/mL时,蛋白质脱除率为74.35%,多糖保留率为80.75%。利用超滤技术将通过径向流后和未通过径向流的铜藻多糖分离为10 ku以下、10～100 ku和100 ku以上各3个组分,通过对比多糖组分的蛋白质脱除率,得出径向流耦合超滤较单一法蛋白质脱除率上都有提高,耦合法脱除率达95%以上。对铜藻多糖各组分的保润性能进行测定,结果显示,铜藻多糖无论在防止解吸还是防止吸湿方面都有提高。

The process parameters of deproteinized of Sargassum Horneris extraction through radial flow chromatography was studied.Sample concentration,sample volume,sample flow-rate and elution flow-rate were studied on the influence of the deproteinization rate and polysaccharide recovery rate.The result showed that in the condition of sample flow-rate 5mL/min,the elution flow-rate 40mL/min,the sample volume 100mL and the sample concentration 5mg/mL,the deproteinization rate was 74.35% and the polysaccharide recovery rate was 80.75%.Furthermore,ultrafiltration was applied to separate the polysaccharides of passed and residues of the radial flow chromatography into three groups: less than 10ku,between 10ku and 100ku and above 100ku.The comparison of protein removal rates from polysaccharides indicated that ultrafiltration coupled radial flow chromatography improved the protein removal rates to 96%.The moisture retention capacity of polysaccharides of Sargassum Horneri indicated that the ability of preventing desorption and moisture was both improved.