研究建立了单层培养,双层显色分离产生物胺肠细菌和乳酸菌的方法,并分离到96株产生物胺肠细菌和58株产生物胺乳酸菌。经变性凝胶梯度电泳分析(PCR-DGGE)和PCR扩增测序后与Genebank数据库比对得知,这154株产胺菌属于5种菌,分别为屎肠球菌、粪肠球菌、阴沟肠杆菌、大肠埃希杆菌和产气肠杆菌。并采用特异性引物证明了产生物胺基因的存在。

A new method of monolayer cultivation coupled with double-layer color development was established.96 strains of biogenic amines-producing lactic acid bacteria and 58 strains of biogenic amines-producing Enterobacteria were obtained.The 154 strains of biogenic amines-producing bacteria belonged to 5 species according to the results of polymerase chain reaction denaturing gradient gel eleetrophoresis(PCR-DGGE) analysis.After sequencing of PCR-products of 16S rDNA fragments and alignment in Genebank,the 5 strains of biogenic amines-producing bacteria were found to be Enterococcus faecium,Enterococcus faecalis,Enterobacter cloacae,Escherichia coli,and Enterobacter aerogenes.The presence of Biogenic amines-producing gene was demonstrated by special primers.